rabbit polyclonal anti calbindin d 28k  (Swant)

Journal: Frontiers in Neuroscience

Article Title: Upregulation of Neural Cell Adhesion Molecule 1 and Excessive Migration of Purkinje Cells in Cerebellar Cortex

doi: 10.3389/fnins.2021.804402

Figure Lengend Snippet: Sagittal sections at P17 of the wt and nax cerebellum. (A) Immunostaining with anti-calbindin (CALB1) in a section through the cerebellum of the wt sibling showing the dendrites of PCs in the molecular layer (ml) and Purkinje cell (PC) soma in the Purkinje cell layer (PCl). (B) In the nax cerebellum showing that the PCs fail to form a uniform monolayer and PCs soma are intermingled in the ml; labeled as pcl/ml. gl, Granular layer; wm, white matter. Scale bar: A = 50 μm; B = 50 μm.

Article Snippet: During immunohistochemistry process, the tissues sections were blocked with NGS (Normal goat serum 10% including 0.3% triton × 100) for an hour at room temperature (RT) and then incubated with the following primary antibodies: Rabbit polyclonal anti-calbindin D-28K antiserum CALB1 (Swant Cat# CB38, RRID:AB_10000340 , 1:5,000), mouse monoclonal anti-S-100 (β-Subunit) antibody (Sigma-Aldrich Cat# S2532, RRID:AB_477499 , 1:500) and mouse monoclonal_anti-NCAM1 (DSHB Cat# 5b8, RRID:AB_528393 , 1:100) overnight at 4°C.

Techniques: Immunostaining, Labeling

Journal: Frontiers in Neuroscience

Article Title: Upregulation of Neural Cell Adhesion Molecule 1 and Excessive Migration of Purkinje Cells in Cerebellar Cortex

doi: 10.3389/fnins.2021.804402

Figure Lengend Snippet: Close relation of BGCs and PCs at P2 and P7 in wt and nax littermate cerebellum. (A–D) Sagittal section of the cerebellum at P2, and P7 were immunofluorescence labeled with anti-calbindin (red) (CALB1; specific marker for PCs) and anti-S100β (green) (specific marker of BGCs). (A,B) Images show multiple layers of PCs and the BGC soma at P2 in both wt (A) and nax (B) cerebellum. The BGC fibers (arrowhead in high magnifications a,b ) are extended from basal end and pass the cluster of PCs’ (arrow in high magnifications a,b ) intercellular space where BGC soma is located. In other word, the extension ends in pial surface and the BGC soma are located in PCs cluster. (C,D) The location of BGCs (arrowhead in high magnifications a,b ) is shown at P7 among PCs in PC layer of wt cerebellum (C) . BGCs and their fibers are located in nax cerebellar sections in PC/molecular layer close to EGZ (D) . The BGC fibers in nax cerebellum are not well organised as in wt cerebellum, but still basal end are connected to the pia matter through their fiber extensions. PCs, Purkinje cells; BGCs, Bergman glia cells; pcl, Purkinje cell layer; ml, Molecular layer. Scale bars: 100 μm (applies to panels A–D ); 50 μm (applied to panels a–d ).

Article Snippet: During immunohistochemistry process, the tissues sections were blocked with NGS (Normal goat serum 10% including 0.3% triton × 100) for an hour at room temperature (RT) and then incubated with the following primary antibodies: Rabbit polyclonal anti-calbindin D-28K antiserum CALB1 (Swant Cat# CB38, RRID:AB_10000340 , 1:5,000), mouse monoclonal anti-S-100 (β-Subunit) antibody (Sigma-Aldrich Cat# S2532, RRID:AB_477499 , 1:500) and mouse monoclonal_anti-NCAM1 (DSHB Cat# 5b8, RRID:AB_528393 , 1:100) overnight at 4°C.

Techniques: Immunofluorescence, Labeling, Marker

Journal: eLife

Article Title: A novel, ataxic mouse model of ataxia telangiectasia caused by a clinically relevant nonsense mutation

doi: 10.7554/eLife.64695

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-Calbindin (D-28k)(Rabbit, polyclonal) , Swant Inc. , Swant Cat# CB 38, RRID: AB_10000340 , IF (1:1000).

Techniques: Mutagenesis, Generated, Sequencing, Staining, Modification, Formulation, Bicinchoninic Acid Protein Assay, Software